X chromosome reactivation in mouse embryonal carcinoma cells.

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Spontaneous reactivation of the inactive X chromosome in mouse embryonal carcinoma cells.

The mouse embryonal carcinoma cell line MC12 carries two X chromosomes, one of which replicates late in S phase and shares properties with the normal inactive X chromosome and, therefore, is considered to be inactivated. Since the hypoxanthine phosphoribosyl transferase (HPRT) gene on the active X chromosome is mutated (HPRT(NDASH;)), MC12 cells lack HPRT activity. After subjecting MC12 cells t...

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X chromosome inactivation during induced differentiation of a female mouse embryonal carcinoma cell line.

P10 is a line of embryonal carcinoma cells with a euploid female karyotype. By making use of variant alleles of X-linked genes and of cytogenetic procedures, we have previously shown that the P10 cells have two genetically active X chromosomes. We show here that P10 cells rapidly differentiate into a cell type resembling extraembryonic endoderm when cultured in the presence of retinoic acid. Th...

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Reactivation of inactive X chromosome in buccal smear of carcinoma of breast

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ژورنال

عنوان ژورنال: Molecular and Cellular Biology

سال: 1985

ISSN: 0270-7306,1098-5549

DOI: 10.1128/mcb.5.10.2705